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KMID : 0364819870250020094
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Korean Journal of Microbiology
1987 Volume.25 No. 2 p.94 ~ p.102
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Characterization of the Folding Structure of 3´-end of Lactate Dehydrogenase A-mRNA Isolated from Hormone Stimulated Rat C(, Glioma cell culture.
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Abstract
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1
Rat liver LDH A-cDNA has been isolated from a A gtll-rat liver cDNA library and pardaily characterized. The size of the isolated rat liver LDH A-cDNA is shown to be 1.6 Kb and restriction enzyme sites for the rat liver LDH A-cDNA are also mapped. 6$2-nadeolde sequence coding for 3´-end of rat liver LDH A-cDNA has been analyzed and compared to the acleodde sequence of the same region of rat C5-gloms cell LDH A-cDNA which has been cloned from the hormonally stimulated cell cultures. The result shows that 177 nucleotide sequences coding for the C-terri al 59-amino adds are Identical but 505 nucleotide sequences of 3´-noutraulated region of the two LDH A-cDNA exhibit characteristic differences In their audeotide sequences. Computer analysis for the folding structures for 3´tad 400 nucleotide sequences of the two LDH A-cDNA shows a pomdbility Implying that the two LDH A-mRNAs Isolated from different tissues of rats may have different half life and therefore their translational efficiency may be different.
It has been previously demonstrated that Isoprotereaol stimulated rat Cs-glloma cell cures pro. dam LDH A-mRNA showing 2 to 3-fold longer half life in comparison to that of nouladuced LDH A-mRNA. The result therefore support for the idea that hormonally stimulated rat Cs-glbma celk may produce LDH A-mRNA containing different audeotide sequences at the 3´-end aontran atcd region by which the hormonally Induced LDH A-mRNA could have more stable secondary mRNA structure in comparison to that of nosinduced LDH A-mRNA.
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